《植物生理学报》 2009, 45(4): 333-339

马槟榔甜蛋白基因(MBL II)的剪切重组和结构分析

顾文亮^1,2, 胡新文¹, 郭建春^2,*

1 海南大学农学院, 海口570228; 2 中国热带农业科学院热带生物技术研究所, 海口571101

通信作者：郭建春；E-mail: jianchunguoh@163.com；Tel: 0898-66987535

摘　要：

马槟榔甜蛋白(mabinlin II)是我国所特有且唯一的植物甜蛋白, 在体外至今没有得到具有甜味的基因表达产物。本文 采用基因工程手段对基因进行剪切重组, 将重组基因构建成植物表达载体转入拟南芥中, 通过RT-PCR检测导入基因的表 达, 同时采用生物信息学方法对MBL II基因及其重组基因进行分析和甜味检测显示, 转基因拟南芥不具有明显的甜味, 但 RT-PCR 的结果显示, MBL II 基因及其重组基因可在转基因的拟南芥中表达。根据生物信息学方法分析结果推测, 导入拟 南芥中的重组马槟榔甜蛋白可能是具有甜味的蛋白。

关键词：马槟榔; 甜蛋白; 重组基因

收稿：2008-12-05   修定：2009-01-15

资助：国家自然科学基金(30760025)。

Reorganization and Structure Analysis of MBL II Gene

GU Wen-Liang^1,2, HU Xin-Wen¹, GUO Jian-Chun^2,*

¹College of Agriculture, Hainan University, Haikou 570228, China; ²Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Corresponding author: GUO Jian-Chun; E-mail: jianchunguoh@163.com; Tel: 0898-66987535

Abstract:

Mabinlin II is the unique plant sweet protein from Capparis masaikai which is only found in China but no sweetness gene expression products in vitro was reported so far. MBL II gene was constructed into plant expression vector, and the recombinant MBL II was transformed into Arabidopsis. Gene expressions in transgenic Arabidopsis were detected by RT-PCR method. MBL II gene and the recombinant MBL II genes were analyzed and predicted by bioinformatics. The transgenic Arabidopsis plants were tested no sweet. But the RT-PCR results showed that MBL II gene and the restructure MBL II genes were expressed in the transgenic Arabidopsis. Bioinformatics analysis also showed that the recombinant mabinlin II proteins maybe a sweet protein.

Key words: Capparis masaikai; sweet protein; recombinant gene